《植物生理学报》 2010, 46(6): 559-563

蝴蝶兰 EST-SSRs 分析

张君毅*, 陈瑞凤

华侨大学生物工程与技术系, 福建厦门361021

通信作者：张君毅；E-mail: zjy0054@yahoo.com.cn；Tel: 0595-22693 329

摘　要：

对蝴蝶兰属 EST 序列进行了 SSR 分析。蝴蝶兰属 EST 总长为 4.5 Mb, 含有 609 个 SSR。SSR 出现频率 7.65%, 平均距 离 7.39 kb, 平均长度为 22.17 bp。单碱基、二碱基和三碱基重复是主要重复类型, 分别占 EST-SSR 总数的 21.67%、40.39% 和 33.50%。A、AG 和 CCG 分别是单碱基、二碱基和三碱基重复中主导重复基元, 分别占 96.21%、58.54% 和 32.25%。设 计引物及检测的结果表明, 蝴蝶兰EST-SSR标记对兰科其他属植物具有一定的通用性。

关键词：蝴蝶兰; SSR; EST; 微卫星

收稿：2010-03-01   修定：2010-04-06

资助：福建省农业科技重点项目(2009N0041)和泉州重点科技项目(2008N40)。

EST-SSRs Analysis of Moth Orchids

ZHANG Jun-Yi*, CHEN Rui-Feng

Department of Bioengineering & Biotechnology, Huaqiao University, Xiamen, Fujian 361021, China

Corresponding author: ZHANG Jun-Yi; E-mail: zjy0054@yahoo.com.cn; Tel: 0595-22693 329

Abstract:

Simple sequence repeats (SSRs) were investigated in expressed sequence tags (EST) of moth orchids. The total length of moth orchids non-redundant ESTs was 4.5 Mb, including 609 SSRs. The frequency of ESTSSRs was 7.65% and the mean distance was 7.39 kb. The mean length of SSRs was 22.17 bp. Mononucleotide, dinucleotide and trinucleotide repeats were major types, whicn accounted for 21.67%, 40.39% and 33.50% in all SSRs, respectively. A, AG and CCG were mainly frequent motifs in mononucleotide, dinucleotide and trinucleotide, accounting for 96.21%, 58.54% and 32.25%, respectively. Primers were designed and detected. The result showed that some of them could be transferable to other genera of orchid.

Key words: moth orchids; SSR; EST; microsatellite